Field trial tests of FKC vaccines against RSIV genotype Megalocytivirus in cage-cultured mandarin fish (Siniperca chuatsi) in an inland reservoir

Megalocytiviruses are one of the most important causative agents in finfish industry in China, Japan and South East Asia. The viruses are mainly composed of ISKNV, RSIV and TRBIV genotypes. Among them, ISKNV genotype isolate is the most important causative agent in mandarin fish industry in South China. Since its first occurrence in mid-1990s in China, no effective drug has been developed to prevent and control this virus until our recent work. In this study, unusual RSIV genotype Megalocytivirus was validated as the causative agent in natural mass mortality of cage-cultured mandarin fish in

Marine teleost ortholog of catalase from rock bream (Oplegnathus fasciatus): Molecular perspectives from genomic organization to enzymatic behavior with respect to its potent antioxidant properties

Catalases are well known antioxidant enzymes that can mainly dismutate hydrogen peroxide into water and oxygen in order to prevent oxidative stress. The complete genomic DNA (gDNA) sequence of the catalase gene from rock bream (Oplegnathus fasciatus) was identified from our custom-constructed BAC genomic DNA library and designated as RbCat. RbCat consists of 13 exons, separated by 12 introns, within a 13,722-bp gDNA sequence. The complete cDNA sequence (3303 bp) of RbCat is comprised of a 1581-bp coding region, encoding a peptide of 527 amino acids (aa) in length, with a predicted molecular m

Genomic characterization, expression analysis, and antimicrobial function of a glyrichin homologue from rock bream, Oplegnathus fasciatus

Antimicrobial peptides are important innate effector molecules, playing a vital role in antimicrobial immunity in all species. Glyrichin is a transmembrane protein and an antibacterial peptide, exerting its functions against a wide range of pathogenic bacteria. In this study, cDNA and a BAC clone harboring the glyrichin gene were identified from rock bream and characterized. Genomic characterization showed that the OfGlyrichin gene exhibited a 3 exon–2 intron structure. OfGlyrichin is a 79-amino-acid protein with a transmembrane domain at 22GFMMGFAVGMAAGAMFGTFSCLR44. Pai

Construction and analysis of experimental DNA vaccines against megalocytivirus

Iridoviruses are large double-stranded DNA viruses with icosahedral capsid. The Iridoviridae family contains five genera, one of which is Megalocytivirus. Megalocytivirus has emerged in recent years as an important pathogen to a wide range of marine and freshwater fish. In this study, we aimed at developing effective genetic vaccines against megalocytivirus affecting farmed fish in China. For this purpose, we constructed seven DNA vaccines based on seven genes of rock bream iridovirus isolate 1 from China (RBIV-C1), a megalocytivirus with a host range that includes Japanese flounder (Paralich

Caspase 3 from rock bream (Oplegnathus fasciatus): Genomic characterization and transcriptional profiling upon bacterial and viral inductions

Caspase 3 is a prominent mediator of apoptosis and participates in the cell death signaling cascade. In this study, caspase 3 was identified (Rbcasp3) and characterized from rock bream (Oplegnathus fasciatus). The full-length cDNA of Rbcasp3 is 2683 bp and contains an open reading frame of 849 bp, which encodes a 283 amino acid protein with a calculated molecular mass of 31.2 kDa and isoelectric point of 6.31. The amino acid sequence resembles the conventional caspase 3 domain architecture, including crucial amino acid residues in the catalytic site and binding pocket. The genomic length of R

A teleostean angiotensinogen from Oplegnathus fasciatus responses to immune and injury challenges

Angiotensinogen (AGT) is the precursor of the renin-angiotensin system and contributes to osmoregulation, acute-phase and immune responses. A full-length cDNA of the AGT (2004 bp with a 1389 bp coding region) was isolated from rock bream (Rb), Oplegnathus fasciatus. The encoded polypeptide of 463 amino acids had a predicted molecular mass of 51.6 kDa. RbAGT possessed a deduced signal peptide of 22 residues upstream of a putative angiotensin I sequence (23NRVYVHPFHL32). RbAGT possessed a specific domain profile and a signature motif which are characteristics of the ser

Molecular characterization and expression analysis of Interferon regulatory factor 5 (IRF-5) in turbot,Scophthalmus maximus

Interferon regulatory factor 5 (IRF-5) plays a role both in the antiviral and inflammatory responses. In this study, we described the structure, mRNA tissue distribution and regulation of anIRF-5gene from turbot,Scophthalmus maximus(SmIRF-5). The gene sequence ofSmIRF-5is 4275 bp long, composed of 9 exons and 8 introns similar to knownIRF-5genes of vertebrates, and encodes a peptide of 487 amino acids. The deduced protein sequence shares the highest identity of ~60-70% with fish IRF-5 and possesses a DNA-binding domain (DBD), a middle region (MR), an IRF association domain (IAD) and a virus

Characterization of a megalocytivirus from cultured rock bream, Oplegnathus fasciatus (Temminck & Schlege), in China

Li Sun

Megalocytivirus belongs to the Iridoviridae family and is known to affect finfish. Megalocytivirus epizootics have been reported to occur in several cultured fish species in China; however, no megalocytivirus associated with rock bream, Oplegnathus fasciatus (Temminck & Schlege), has been documented. In this study, we characterized for the first time a megalocytivirus, rock bream iridovirus (RBIV)-C1, detected in cultured rock bream in a fish farm in China that had been inflicted with a high-mortality disease outbreak. Sequence analysis of three conserved g

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